Nanofibrous bicomponent scaffolds for the dual delivery of NGF and GDNF: controlled release of growth factors and their biological effects

Abstract Electrospun fibrous scaffolds capable of providing dual growth factor delivery in a controlled manner have distinctive advantages for tissue engineering. In this study, we investigated the formation, structure, and characteristics/properties bicomponent glial cell line-derived neurotrophic (GDNF) nerve (NGF) peripheral regeneration. GDNF NGF were incorporated into core-shell structured poly(lactic-co-glycolic acid) (PLGA) poly ( d , l -lactic (PDLLA) nanofibers, respectively, through emulsion electrospinning. Using dual-source dual-power electrospinning, composed GDNF/PLGA fibers NGF/PDLLA with different fiber component ratios produced. The properties, vitro release behavior mono- systematically investigated. Concurrent sustained from was achieved their profiles could be tuned. biological investigations conducted. Rat pheochromocytoma cells found to attach, spread, proliferate on all scaffolds. factors induce much improved neurite outgrowth neural differentiation. released scaffolds, dose-dependent differentiation separately. exerted synergistic effect promoting